HPLC - Electrospray ionization mass spectrometric analysis of antimalarial drug artemisinin

被引：23

作者：

Sahai, P

Vishwakarma, RA

Bharel, S

Gulati, A

Abdin, MZ

Srivastava, PS

Jain, SK

机构：

[1] Natl Inst Immunol, Bioorgan Chem Lab, New Delhi 110067, India

[2] Hamdard Univ, Dept Biochem, New Delhi 110062, India

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 14期

关键词：

D O I：

10.1021/ac970944f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new analytical method for high-sensitivity direct detection, quantitation, and isotopomer analysis of antimalarial drug artemisinin (qinghaosu) is described using HPLC-electrospray ionization mass spectrometry, without pre- or postcolumn derivatization. The method has been shown to be particularly suitable for analysis of artemisinin in biosynthetic stable isotope labeling experiments and quantitative analysis in the nanogram range in crude biological samples such as Artemisia annua plant extracts. The method enabled isotopomer analysis in biosynthetic O-18-labeling experiments with plant cell-free enzyme preparation.

引用

页码：3084 / 3087

页数：4

共 14 条

[1] REDUCTIVE ELECTROCHEMICAL HPLC ASSAY FOR ARTEMISININ (QINGHAOSU) [J].