In vivo regulation of MAP kinases in Ratus norvegicus renal papilla by water loading and restriction

被引:24
作者
Wojtaszek, PA [1 ]
Heasley, LE [1 ]
Berl, T [1 ]
机构
[1] Univ Colorado, Sch Med, Dept Med, Denver, CO 80262 USA
关键词
heat shock proteins; urine; kidney medulla; kidney cortex; hypertonic solutions;
D O I
10.1172/JCI4384
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
In cultured renal cells, hypertonicity activates multiple mitogen-activated protein kinases (MAPKs) and enhances the expression of heat shock proteins (HSPs). In rats, 24 h water restriction increased mean urinary osmolality (U-osm) from 2,179+/-153 mOsm/kg to 2,944+/-294 mOsm/kg (P < 0.001) and was associated with significant (P < 0.05) increases in the papillary activity of c-Jun NH2-terminal protein kinase (JNK) by 22%, extracellular signal-regulated protein kinase (ERK) by 49%, and p38 MAPK by 15%. Conversely, 24 h of water-loading (U-osm 473+/-33 mOsm/kg) caused suppression of JNK activity by 43% (P < 0.001), ERK by 39% (P < 0.05), and p38 MAPK by 26% (P < 0.05). No such modulation was observed in the isotonic cortex, c-Jun phosphorylation was decreased in papilla from water-loaded rats by 45% versus controls. Expression of Hsp 110, inducible Hsp 70, and Hsp 25 was greater in the hyperosmotic papilla than the isosmotic cortex but was not affected by the animal's hydration state. In cultured inner medullary collecting duct cells, HSP expression was maximal at 500 mOsm/kg, while activation of JNK continued to increase. We conclude that under basal conditions of hydration, these HSPs are maximally expressed in the hypertonic inner medulla, while the activation of all three members of the MAPK family approaches but is not maximal.
引用
收藏
页码:1874 / 1881
页数:8
相关论文
共 28 条
  • [11] REGULATION OF RENAL-CELL ORGANIC OSMOLYTE TRANSPORT BY TONICITY
    HANDLER, JS
    KWON, HM
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (06): : C1449 - C1455
  • [12] HORMONAL-REGULATION OF MAP KINASE IN CULTURED RAT INNER MEDULLARY COLLECTING TUBULE CELLS
    HEASLEY, LE
    SENKFOR, SI
    WINITZ, S
    STRASHEIM, A
    TEITELBAUM, I
    BERL, T
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (03): : F366 - F373
  • [13] MITOGEN-ACTIVATED PROTEIN-KINASE AND ITS ACTIVATOR ARE REGULATED BY HYPERTONIC STRESS IN MADIN-DARBY CANINE KIDNEY-CELLS
    ITOH, T
    YAMAUCHI, A
    MIYAI, A
    YOKOYAMA, K
    KAMADA, T
    UEDA, N
    FUJIWARA, Y
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (06) : 2387 - 2392
  • [14] Characterization of the structure and function of a new mitogen-activated protein kinase (p38 beta)
    Jiang, Y
    Chen, CH
    Li, ZJ
    Guo, W
    Gegner, JA
    Lin, SC
    Han, JH
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (30) : 17920 - 17926
  • [15] Osmotic stress protein 94 (Osp94) - A new member of the Hsp110/SSE gene subfamily
    Kojima, R
    Randall, J
    Brenner, BM
    Gullans, SR
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (21) : 12327 - 12332
  • [16] Distinct regulation of osmoprotective genes in yeast and mammals - Aldose reductase osmotic response element is induced independent of p38 and stress-activated protein kinase Jun N-terminal kinase in rabbit kidney cells
    Kultz, D
    GarciaPerez, A
    Ferraris, JD
    Burg, MB
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) : 13165 - 13170
  • [17] Heat shock proteins HSP25, HSP60, HSP72, HSP73 in isoosmotic cortex and hyperosmotic medulla of rat kidney
    Muller, E
    Neuhofer, W
    Ohno, A
    Rucker, S
    Thurau, K
    Beck, FX
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1996, 431 (04): : 608 - 617
  • [18] AN OSMOTICALLY TOLERANT INNER MEDULLARY COLLECTING DUCT CELL-LINE FROM AN SV40 TRANSGENIC MOUSE
    RAUCHMAN, MI
    NIGAM, SK
    DELPIRE, E
    GULLANS, SR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (03): : F416 - F424
  • [19] Induction of molecular chaperones by hyperosmotic stress in mouse inner medullary collecting duct cells
    Rauchman, MI
    Pullman, J
    Gullans, SR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1997, 273 (01) : F9 - F17
  • [20] Characterization of the Hsp110/SSE gene family response to hyperosmolality and other stresses
    Santos, BC
    Chevaile, A
    Kojima, R
    Gullans, SR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1998, 274 (06) : F1054 - F1061