Real-time imaging of type III secretion:: Salmonella SipA injection into host cells

被引：162

作者：

Schlumberger, MC ^{[1
]}

Müller, AJ ^{[1
]}

Ehrbar, K ^{[1
]}

Winnen, B ^{[1
]}

Duss, I ^{[1
]}

Stecher, B ^{[1
]}

Hardt, WD ^{[1
]}

机构：

[1] ETH, Inst Microbiol, CH-8093 Zurich, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 35期

关键词：

effector protein; time-lapse microscopy;

D O I：

10.1073/pnas.0503407102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many pathogenic and symbiotic Gram-negative bacteria employ type III secretion systems to inject "effector" proteins into eukaryotic host cells. These effectors manipulate signaling pathways to initiate symbiosis or disease. By using time-lapse microscopy, we have imaged delivery of the Salmonella type III effector protein SipA/SspA into animal cells in real time. SipA delivery mostly began 10-90 sec after docking and proceeded for 100-600 sec until the bacterial SipA pool (6 +/- 3 x 10(3) molecules) was exhausted. Similar observations were made for the effector protein SopE. This visualization of type III secretion in real time explains the efficiency of host cell manipulation by means of this virulence system.

引用

页码：12548 / 12553

页数：6

共 30 条

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