Fluorimetric determination of diarrhetic shellfish toxins in scallops and mussels by high-performance liquid chromatography

被引：16

作者：

Akasaka, K ^{[1
]}

Ohrui, H ^{[1
]}

Meguro, H ^{[1
]}

Yasumoto, T ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT APPL BIOL CHEM,AOBA KU,SENDAI,MIYAGI 981,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 729卷 / 1-2期

关键词：

diarrhetic shellfish poisoning; okadaic acid; dinophysistoxin-1; toxins;

D O I：

10.1016/0021-9673(95)00890-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorimetric determination of okadaic acid (OA) and dinophysistoxin-1 (DTX-1), the principal toxins of diarrhetic poisoning, is reported. The digestive glands of mussels or scallops were homogenized with 2-propanol. OA and DTX-1 were extracted from the homogenate, with hexane-ethyl acetate and labelled with 2,3-(anthracenedicarboximido)ethyl trifluoromethanesulfonate in dry acetonitrile. After cleaning up by passage through a short silica gel column, the fluorescent derivatives were determined by HPLC. The derivatives were at first separated on a Develosil Ph-5 column, and only the target fraction obtained was introduced into a Develosil ODS K-5 column by a valve-switching device. Both toxins were determined in the range 2.5-500 pg, and the detection limits were 0.8 pg (OA) and 1.3 pg (DTX-1) with a signal-to-noise ratio of 3.

引用

页码：381 / 386

页数：6

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