Successful outcome with day 4 embryo transfer after preimplantation diagnosis for genetically transmitted diseases

被引:29
作者
Grifo, JA [1 ]
Giatras, K [1 ]
Tang, YX [1 ]
Krey, LC [1 ]
机构
[1] NYU Med Ctr, Dept Obstet & Gynecol, Div Reprod Endocrinol, New York, NY 10016 USA
关键词
embryo biopsy; polymerase chain reaction; preimplantation diagnosis;
D O I
10.1093/humrep/13.6.1656
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Preimplantation genetic diagnosis was performed in 61 day 3 embryos obtained by in-vitro fertilization from seven patient carriers of haemophilia, Marfan's syndrome, Bloch-Sulzemberg syndrome (incontinentia pigmentosa) or X chromosome-linked immune deficiency, retinitis pigmentosa, and FG syndrome, which is characterized by mental retardation and hypotonia. After multiplex polymerase chain reaction, 16 embryos were diagnosed as being unaffected, and these were transferred to the uterus on the following day (day 4). Of these embryos, six (37.5%) implanted, resulting in the delivery of a singleton and a twin pregnancy a late second trimester miscarriage (twins at week 20) and a first trimester miscarriage at week 8. All the diagnoses mere confirmed by amniocentesis. We report for the first time a late day 4 transfer of biopsied human embryos undergoing preimplantation genetic diagnosis. This transfer schedule allows an extra day to perform genetic analyses on single blastomeres and to monitor any adverse effect of the biopsy procedure.
引用
收藏
页码:1656 / 1659
页数:4
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