Novel heme ligand displacement by CO in the soluble hemophore HasA and its proximal ligand mutants: Implications for heme uptake and release

被引:33
作者
Lukat-Rodgers, Gudrun S.
Rodgers, Kenton R.
Caillet-Saguy, Celia
Izadi-Pruneyre, Nadia
Lecroisey, Anne
机构
[1] N Dakota State Univ, Dept Chem Biochem & Mol Biol, Fargo, ND 58105 USA
[2] Inst Pasteur, CNRS, URA 2185, Unite Resonance Magnet Nucl Biomol, Paris, France
关键词
D O I
10.1021/bi7019518
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
HasA(SM), a hemophore secreted by the Gram-negative bacteria Serratia marcescens, extracts heme from host hemoproteins and shuttles it to HasR(SM), a specific hemophore outer membrane receptor. Heme iron in HasA(SM) is in a six-coordinate ferric state. It is linked to the protein by the heretofore uncommon axial ligand set, His32 and Tyr75. A third residue of the heme pocket, His83, plays a crucial role in heme ligation through hydrogen bonding to Tyr75. The vibrational frequencies of coordinated carbon monoxide constitute a sensitive probe of trans ligand field, FeCO structure, and electrostatic landscape of the distal heme pockets of heme proteins. In this study, carbonyl complexes of wild-type (WT) HasA(SM) and its heme pocket mutants HiS32Ala, Tyr75Ala, and His83Ala were characterized by resonance Raman spectroscopy. The CO complexes of WT HasA(SM), HasA(SM)(His32Ala), and HasA(SM)(His83Ala) exhibit similar spectral features and fall above the line that correlates v(Fe-CO) and v(C-O) for proteins having a proximal imidazole ligand. This suggests that the proximal ligand field in these CO adducts is weaker than that for heme-CO proteins bearing a histidine axial ligand. In contrast, the CO complex of HasA(SM)(Tyr75Ala) has resonance Raman signatures consistent with ImH-Fe-CO ligation. These results reveal that in WT HasA(SM), the axial ImH side chain of His32 is displaced by CO. This is in contrast to other heme proteins known to have the His/Tyr axial ligand set, wherein the phenolic side chain of the Tyr ligand dissociates upon CO addition. The displacement of His32 and its stabilization in an unbound state is postulated to be relevant to heme uptake and/or release.
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页码:2087 / 2098
页数:12
相关论文
共 60 条
[41]   Interactions of soluble guanylate cyclase with diatomics as probed by resonance Raman spectroscopy [J].
Pal, B ;
Kitagawa, T .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2005, 99 (01) :267-279
[42]   Functional implications of the proximal hydrogen-bonding network in myoglobin: A resonance Raman and kinetic study of Leu89, Ser92, His97, and F-helix swap mutants [J].
Peterson, ES ;
Friedman, JM ;
Chien, EYT ;
Sligar, SG .
BIOCHEMISTRY, 1998, 37 (35) :12301-12319
[43]   Active and inhibited human catalase structures: Ligand and NADPH binding and catalytic mechanism [J].
Putnam, CD ;
Arvai, AS ;
Bourne, Y ;
Tainer, JA .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 296 (01) :295-309
[44]   HOW FAR CAN PROTEINS BEND THE FECO UNIT - DISTAL POLAR AND STERIC EFFECTS IN HEME-PROTEINS AND MODELS [J].
RAY, GB ;
LI, XY ;
IBERS, JA ;
SESSLER, JL ;
SPIRO, TG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (01) :162-176
[45]   Identification and characterization of the hemophore-dependent heme acquisition system of Yersinia pestis [J].
Rossi, MS ;
Fetherston, JD ;
Létoffé, S ;
Carniel, E ;
Perry, RD ;
Ghigo, JM .
INFECTION AND IMMUNITY, 2001, 69 (11) :6707-6717
[46]   SOUL in mouse eyes is a new hexameric heme-binding protein with characteristic optical absorption, resonance Raman spectral, and heme-binding properties [J].
Sato, E ;
Sagami, I ;
Uchida, T ;
Sato, A ;
Kitagawa, T ;
Igarashi, J ;
Shimizu, T .
BIOCHEMISTRY, 2004, 43 (44) :14189-14198
[47]  
SHARMA KD, 1989, J BIOL CHEM, V264, P12772
[48]   CYTOCHROME-C PEROXIDASE MUTANT ACTIVE-SITE STRUCTURES PROBED BY RESONANCE RAMAN AND INFRARED SIGNATURES OF THE CO ADDUCTS [J].
SMULEVICH, G ;
MAURO, JM ;
FISHEL, LA ;
ENGLISH, AM ;
KRAUT, J ;
SPIRO, TG .
BIOCHEMISTRY, 1988, 27 (15) :5486-5492
[49]   CO as a vibrational probe of heme protein active sites [J].
Spiro, TG ;
Wasbotten, IH .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2005, 99 (01) :34-44
[50]   HEME-HEME OXYGENASE COMPLEX - STRUCTURE AND PROPERTIES OF THE CATALYTIC SITE FROM RESONANCE RAMAN-SCATTERING [J].
TAKAHASHI, S ;
WANG, JL ;
ROUSSEAU, DL ;
ISHIKAWA, K ;
YOSHIDA, T ;
TAKEUCHI, N ;
IKEDASAITO, M .
BIOCHEMISTRY, 1994, 33 (18) :5531-5538