Removal of inhibitors of CSF-PCR to improve diagnosis of herpesviral encephalitis

被引：19

作者：

Ratnamohan, VM

Cunningham, AL

Rawlinson, WD

机构：

[1] Westmead Hosp, ICPMR, Ctr Infect Dis, Dept Virol, Westmead, NSW 2145, Australia

[2] Westmead Hosp, ICPMR, Microbiol Lab, Westmead, NSW 2145, Australia

[3] Westmead Hosp, Ctr Virus Res, Westmead Inst Hlth Res, Westmead, NSW 2145, Australia

[4] Univ Sydney, Westmead, NSW 2145, Australia

[5] Prince Wales Hosp, Dept Microbiol, Div Virol, Randwick, NSW 2031, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 72卷 / 01期

关键词：

CSF-PCR; inhibitors; herpes simplex virus; cytomegalovirus;

D O I：

10.1016/S0166-0934(98)00020-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

CSF-PCR is currently the most sensitive test to diagnose encephalitis due to cytomegalovirus or herpes simplex virus. However, false negative results sometimes arise due to inhibitors in CSF. We have shown that the inhibitory effects may be due to increased levels of proteins and increased cell numbers, but are not due to cellular DNA. Simple techniques were used to remove the inhibitors and successfully apply these methods to CSF specimens that gave equivocal results when tested untreated. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：59 / 65

页数：7

共 17 条

[11] RAPID AND SENSITIVE PROTEIN SIMILARITY SEARCHES [J].