Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas

被引:119
作者
Gulati, Arvind [1 ]
Rahi, Praveen [1 ]
Vyas, Pratibha [1 ]
机构
[1] Inst Himalayan Bioresource Technol, Palampur 176061, Himachal Prades, India
关键词
D O I
10.1007/s00284-007-9042-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Isolation and characterization of fluorescent pseudomonads with high phosphate-solubilizing ability is reported from the alkaline and calcium-rich soils with low P availability in the cold desert region of Lahaul and Spiti in the trans-Himalayas of India. Of 216 phosphate-solubilizing isolates, 12 exhibiting high solubilization of tricalcium phosphate (TCP) in NBRIP liquid culture were identified as Pseudomonas trivialis, P. poae, P. fluorescens, and Pseudomonas spp. on the basis of phenotypic features, whole-cell fatty acids methyl ester (FAME) profiles, and 16S rDNA sequencing. These isolates also showed relatively high solubilization of North Carolina rock phosphate (NCRP) in comparison to the solubilization of Mussoorie rock phosphate (MRP) and Udaipur rock phosphate (URP). The solubilization of phosphate substrates by P. trivialis and P. poae is reported for the first time.
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页码:73 / 79
页数:7
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