Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae

被引：22

作者：

Vyskocilova, Martina

Simkova, Andrea

Martin, Jean-Francois

机构：

[1] Masaryk Univ, Fac Sci, Inst Bot & Zool, Brno 61137, Czech Republic

[2] Acad Sci Czech Republic, Inst Vertebrate Biol, Brno 60365, Czech Republic

[3] Ctr Biol & Gest Populat, Montpellier SupAgro, F-34988 Montferrier Sur Lez, France

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期

关键词：

cross-amplification; Cyprinidae; Leuciscus; microsatellites; primers;

D O I：

10.1111/j.1471-8286.2007.01813.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thirteen polymorphic microsatellites were isolated from Leuciscus cephalus, a widespread cyprinid species with great ecological tolerance. Together with the cross-species amplification of six additional loci originally published for three cyprinid fish species, we optimized a multiplex panel for L. cephalus allowing the genotyping of 19 polymorphic loci. Number of alleles and heterozygosity per locus in a sample of 20 fish individuals ranged from two to 16 and from 0.05 to 0.90, respectively. These primers will be useful in determining the population structure of L. cephalus. In addition, successful cross-amplification was obtained for several species of Cyprinidae.

引用

页码：1150 / 1154

页数：5

共 10 条

[1] Microsatellite null alleles and estimation of population differentiation [J].