Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae

被引：28

作者：

Oldfield, Neil J. ^{[1
]}

Donovana, Elizabeth A. ^{[1
]}

Worrall, Kathryn E. ^{[1
]}

Wooldridge, Karl G. ^{[1
]}

Langford, Paul R. ^{[2
]}

Rycroft, Andrew N. ^{[3
]}

Ala'Aldeen, Dlawer A. A. ^{[1
]}

机构：

[1] Univ Nottingham, Mol Bacteriol & Immunol Grp, Inst Infect Immun & Inflammat, Ctr Biomol Sci, Nottingham NG7 2RD, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Paediat, Div Med, London W2 1PG, England

[3] Univ London Royal Vet Coll, Dept Infect & Infect Dis, N Mymms AL9 7TA, Herts, England

来源：

VACCINE | 2008年 / 26卷 / 16期

基金：

英国生物技术与生命科学研究理事会;

关键词：

A; pleuropneumoniae; outer membrane proteins; vaccine;

D O I：

10.1016/j.vaccine.2008.02.022

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine components. Open reading frames within immuno-reactive phage were analyzed in silica to identify conserved outer rnembrane proteins. Four ORFs, named comL, lolB, IppC and ompA were chosen for further study. The four encoded proteins were shown experimentally to be antigenic, highly conserved, outer membrane, in vivo-expressed proteins. In pig protection studies, none of the proteins was individually capable of protecting pigs from colonization and infection with the homologous A. pleuropneumoniae strain, despite a detectable specific antibody response being induced. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1942 / 1954

页数：13

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