Identification of Non-diphtheriae Corynebacterium by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引:75
作者
Alatoom, Adnan A. [1 ]
Cazanave, Charles J. [1 ]
Cunningham, Scott A. [1 ]
Ihde, Sherry M. [1 ]
Patel, Robin [1 ,2 ]
机构
[1] Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
[2] Mayo Clin, Dept Med, Div Infect Dis, Rochester, MN USA
关键词
ENDOCARDITIS; AURIMUCOSUM; RPOB;
D O I
10.1128/JCM.05889-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of >= 1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
引用
收藏
页码:160 / 163
页数:4
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