Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Routine Identification of Yeast

被引：219

作者：

Dhiman, Neelam ^{[1
]}

Hall, Leslie ^{[1
]}

Wohlfiel, Sherri L. ^{[1
]}

Buckwalter, Seanne P. ^{[1
]}

Wengenack, Nancy L. ^{[1
]}

机构：

[1] Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

SPECIES IDENTIFICATION; RAPID IDENTIFICATION; CANDIDA-ALBICANS; RIBOSOMAL DNA; BACTERIA; VITEK-2; REGION; EXPERIENCE; REVOLUTION; SYSTEMS;

D O I：

10.1128/JCM.02381-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, >= 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.

引用

页码：1614 / 1616

页数：3

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