Expression of the S receptor kinase in self-compatible Brassica napus cv.: Westar leads to the allele-specific rejection of self-incompatible Brassica napus pollen

被引:74
作者
Silva, NF
Stone, SL
Christie, LN
Sulaman, W
Nazarian, KAP
Burnett, LA
Arnoldo, MA
Rothstein, SJ
Goring, DR
机构
[1] York Univ, Dept Biol, N York, ON M3J 1P3, Canada
[2] Pioneer Hi Bred Prod Ltd, Canola Res Stn, Georgetown, ON L7G 4S7, Canada
[3] Pioneer HiBred Int Inc, Trait & Technol Dev, Johnston, IA 50131 USA
关键词
Brassica napus; S locus receptor kinase (SRK); S locus glycoprotein (SLG); self-incompatibility; signal transduction;
D O I
10.1007/s004380100446
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Expression of an S receptor kinase (SRK910) transgene in self-compatible Brassica napus cr. Westar conferred on the transgenic pistil the ability to reject pollen from the self-incompatible Brassica napus W1 line, which carries the S-910 allele. In one of the SRK transgenic lines, 1C, virtually no seeds were produced when the transgenic pistils were pollinated with W1 pollen (Mean number of seeds per pod = 1.22). This response was specific to the W1 pollen since pollen from a different self-incompatible Brassica napus line (T2) and self-pollinations were fully compatible. Westar plants expressing an S locus glycoprotein transgene (SLG(910)) did not show any self-incompatibility response towards W1 pollen. Transgenic Westar plants resulting from crosses between the 1C SRK transgenic line and three SLG(910) transgenic lines were also tested for rejection of W1 pollen. The additional expression of the SLG(910) transgene in the SRK910 transgenic plants did not cause any significant further reduction in seed production (Mean seeds/pod = 1.04) or have any detectable effects the number of pollen grains that adhered to the pistil. Thus, while the allele-specific SLG gene was previously reported to have an enhancing effect on the self-incompatibility response, no evidence for such a role was found in this study.
引用
收藏
页码:552 / 559
页数:8
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