Characterization of Rhizobium loti strains from the Salado River Basin

被引：12

作者：

Fulchieri, MM ^{[1
]}

Estrella, MJ ^{[1
]}

Iglesias, AA ^{[1
]}

机构：

[1] Inst Tecnol Chascomus, Inst Invest Biotecnol, RA-7130 Buenos Aires, DF, Argentina

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2001年 / 79卷 / 02期

关键词：

Lotus spp; RFLP-PCR; Rhizobium loti; symbiotic interaction;

D O I：

10.1023/A:1010221208619

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Thirty indigenous rhizobia strains, isolated from Lotus tenuis in the area of Chascomus and other regions of the Salado River Basin (Argentina), were characterized based on generation time, acid production, carbon utilization, protein profile, and molecular characterization by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes amplified by the polymerase chain reaction (PCR). The results indicated that native rhizobia isolates from the Chascomus area are predominantly fast and intermediate-growers. The unclassified rhizobia examined by PCR-RFLP were found to be closely related to the reference strains of validly described Rhizobium species.

引用

页码：119 / 125

页数：7