Determination of thermodynamic and kinetic properties of biomolecules by mass spectrometry

被引：30

作者：

Guelbakan, Basri ^{[2
,3
]}

Barylyuk, Konstantin ^{[2
]}

Zenobi, Renato ^{[1
,2
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[2] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[3] Hacettepe Univ, Inst Child Hlth, Div Pediat Basic Sci, TR-06100 Ankara, Turkey

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 31卷

关键词：

ELECTRON-CAPTURE DISSOCIATION; PROTEIN-LIGAND BINDING; OF-FLIGHT INSTRUMENT; H/D EXCHANGE; HYDROGEN-EXCHANGE; GAS-PHASE; HYDROGEN/DEUTERIUM EXCHANGE; UNPURIFIED PROTEINS; SUPREX STABILITY; IONIZATION;

D O I：

10.1016/j.copbio.2014.08.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the past two decades, mass spectrometry (MS) has transformed the life sciences. The advances in understanding biomolecule structure and function by MS is progressing at an accelerated pace. MS has also largely been applied to study thermodynamic and kinetic structure of biomolecules. Herein, we highlight the recent discussions about native mass spectrometry and studies about determining stable gas phase structures, hydrogen/deuterium exchange studies about reaction kinetics and determination of binding constants of biomolecules with their ligands.

引用

页码：65 / 72

页数：8

共 68 条

[1] A New Approach to Measuring Protein Backbone Protection with High Spatial Resolution Using H/D Exchange and Electron Capture Dissociation [J].

Abzalimov, Rinat R. ;

Bobst, Cedric E. ;

Kaltashov, Igor A. .

ANALYTICAL CHEMISTRY, 2013, 85 (19) :9173-9180

[2] Protein Conformations Can Be Probed in Top-Down HDX MS Experiments Utilizing Electron Transfer Dissociation of Protein Ions Without Hydrogen Scrambling [J].

Abzalimov, Rinat R. ;

Kaplan, Desmond A. ;

Easterling, Michael L. ;

Kaltashov, Igor A. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2009, 20 (08) :1514-1517

[3] DNA Oligonucleotides: A Model System with Tunable Binding Strength to Study Monomer-Dimer Equilibria with Electrospray Ionization-Mass Spectrometry [J].

Barylyuk, Konstantin ;

Guelbakan, Basri ;

Xie, Xueshu ;

Zenobi, Renato .

ANALYTICAL CHEMISTRY, 2013, 85 (24) :11902-11912

[4]

Bernstein SL, 2009, NAT CHEM, V1, P326, DOI [10.1038/nchem.247, 10.1038/NCHEM.247]

[5]

Bleiholder C, 2011, NAT CHEM, V3, P172, DOI [10.1038/nchem.945, 10.1038/NCHEM.945]

[6] Electrospray ionization of a whole virus: Analyzing mass, structure, and viability [J].

Bothner, B ;

Siuzdak, G .

CHEMBIOCHEM, 2004, 5 (03) :258-260

[7] Nonresonant femtosecond laser vaporization of aqueous protein preserves folded structure [J].

Brady, John J. ;

Judge, Elizabeth J. ;

Levis, Robert J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (30) :12217-12222

[8] Native electron capture dissociation for the structural characterization of noncovalent interactions in native cytochrome c [J].

Breuker, K ;

McLafferty, FW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (40) :4900-4904

[9] Femtosecond laser vaporization that preserves protein-folded structure: An unproven idea [J].

Breuker, Kathrin ;

Skinner, Owen S. ;

McLafferty, Fred W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (05) :E206-E206

[10] Stepwise evolution of protein native structure with electrospray into the gas phase, 10-12 to 102 S [J].

Breuker, Kathrin ;

McLafferty, Fred W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18145-18152

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