Detoxification of aflatoxin B1 by enzymes isolated from Armillariella tabescens

被引：91

作者：

Liu, DL

Yao, DS

Liang, R

Ma, L

Cheng, WQ

Gu, LQ

机构：

[1] GuangDong Coll Pharm, Inst Pharm, Guangzhou 510224, Peoples R China

[2] Sun Yat Sen Univ, Dept Appl Chem, Guangzhou 510275, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 1998年 / 36卷 / 07期

关键词：

Armillariella tabescens; fungal multienzyme; aflatoxin B-1; detoxification;

D O I：

10.1016/S0278-6915(98)00017-9

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Detoxification of aflatoxin B-1 (AFB(1)) by Armillariella tabescens multienzyme, which was isolated from mycelium pellets of A. tabescens, was confirmed by thin-layer chromatography (TLC) and rat assay. The results of toxicology and pathology studies showed that toxicity of AFB(1) was minimized after treatment with A. tabescens multienzyme. The result of the Ames test indicated that the mutagenic activity of multienzyme-treated AFB(1) was greatly reduced (or inactivated) compared with that of untreated controls. TLC determinations showed that AFB(1) at an initial concentration of 16 mu M was completely detoxified (100%) by the fungal multienzyme. The infrared spectrum suggests that the multienzyme is responsible for opening the difuran ring of AFB(1). (C) 1998 Elsevier Science Lid. All rights reserved.

引用

页码：563 / +

页数：11

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