Proteome analysis: Biological assay or data archive?

被引：123

作者：

Haynes, PA ^{[1
]}

Gygi, SP ^{[1
]}

Figeys, D ^{[1
]}

Aebersold, R ^{[1
]}

机构：

[1] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 11期

关键词：

proteome; two-dimensional polyacrylamide gel electrophoresis; tandem mass spectrometry;

D O I：

10.1002/elps.1150191104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this review we examine the current state of proteome analysis. There are three main issues discussed: why it is necessary to study proteomes; how proteomes can be analyzed with current technology; and how proteome analysis can be used to enhance biological research. We conclude that proteome analysis is an essential tool in the understanding of regulated biological systems. Current technology, while still mostly limited to the more abundant proteins, enables the use of proteome analysis both to establish databases of proteins present, and to perform biological assays involving measurement of multiple variables. We believe that the utility of proteome analysis in future biological research will continue to be enhanced by further improvements in analytical technology.

引用

页码：1862 / 1871

页数：10

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