Impedance spectroscopy technique for DNA hybridization

被引：39

作者：

Hleli, S

Abdelghani, A ^{[1
]}

Tlili, A

机构：

[1] IPEST, Unite Rech Phys Semicond, Tunis 2070, Tunisia

[2] Inst Natl Sci Appl & Technol, Ctr Urbain Nord, Charguia 1080, Tunisia

来源：

SENSORS | 2003年 / 3卷 / 10期

关键词：

impedance spectroscopy; DNA; oligonucleotide;

D O I：

10.3390/s31000472

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target. This article describes the use of impedance spectroscopy technique for digoxigeninthiol-labeled ssDNA probe immobilization, anti-digoxigenin binding and targeted DNA hybridization. The analysis of the impedance spectra in terms of equivalents circuits of the gold/electrolyte interface and gold/digoxigenin-thiol-labeled/electrolyte interface is discussed. The DNA hybridization shows a variation in the impedance spectra.

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收藏

页码：472 / 479

页数：8

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