A highly conserved 6S RNA structure is required for regulation of transcription

被引:136
作者
Trotochaud, AE [1 ]
Wassarman, KM [1 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
关键词
D O I
10.1038/nsmb917
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
6S RNA, a highly abundant noncoding RNA, regulates transcription through interaction with RNA polymerase in Escherichia coli. Computer searches identified 6S RNAs widely among gamma-proteobacteria. Biochemical approaches were required to identify more divergent 6S RNAs. Two Bacillus subtilis RNAs were found to interact with the housekeeping form of RNA polymerase, thereby establishing them as 6S RNAs. A third B. subtilis RNA was discovered with distinct RNA polymerase - binding activity. Phylogenetic comparison and analysis of mutant RNAs revealed that a conserved secondary structure containing a single-stranded central bulge within a highly double-stranded molecule was essential for 6S RNA function in vivo and in vitro. Reconstitution experiments established the marked specificity of 6S RNA interactions for sigma(70)-RNA polymerase, as well as the ability of 6S RNA to directly inhibit transcription. These data highlight the critical importance of structural characteristics for 6S RNA activity.
引用
收藏
页码:313 / 319
页数:7
相关论文
共 32 条
[1]   The SINE-encoded mouse B2 RNA represses mRNA transcription in response to heat shock [J].
Allen, TA ;
Von Kaenel, S ;
Goodrich, JA ;
Kugel, JF .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (09) :816-821
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   A small, stable RNA induced by oxidative stress: Role as a pleiotropic regulator and antimutator [J].
Altuvia, S ;
WeinsteinFischer, D ;
Zhang, AX ;
Postow, L ;
Storz, G .
CELL, 1997, 90 (01) :43-53
[4]   Expression of a small RNA, BS203 RNA, from the yocI-yocJ intergenic region of Bacillus subtilis genome [J].
Ando, Y ;
Asari, S ;
Suzuma, S ;
Yamane, K ;
Nakamura, K .
FEMS MICROBIOLOGY LETTERS, 2002, 207 (01) :29-33
[5]  
Anthony LC, 2003, METHOD ENZYMOL, V370, P181
[6]   RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro [J].
Artsimovitch, I ;
Svetlov, V ;
Anthony, L ;
Burgess, RR ;
Landick, R .
JOURNAL OF BACTERIOLOGY, 2000, 182 (21) :6027-6035
[7]   Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase [J].
Breyer, MJ ;
Thompson, NE ;
Burgess, RR .
JOURNAL OF BACTERIOLOGY, 1997, 179 (04) :1404-1408
[8]   SEQUENCE OF 6S RNA OF E-COLI [J].
BROWNLEE, GG .
NATURE-NEW BIOLOGY, 1971, 229 (05) :147-&
[9]   B2 RNA binds directly to RNA polymerase II to repress transcript synthesis [J].
Espinoza, CA ;
Allen, TA ;
Hieb, AR ;
Kugel, JF ;
Goodrich, JA .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (09) :822-829
[10]   The small RNA regulators of Escherichia coli:: Roles and mechanisms [J].
Gottesman, S .
ANNUAL REVIEW OF MICROBIOLOGY, 2004, 58 :303-328