Gold nanoparticle aggregation-based highly sensitive DNA detection using atomic force microscopy

被引:21
作者
Bui, Minh-Phuong Ngoc [1 ]
Baek, Taek Jin [1 ]
Seong, Gi Hun [1 ]
机构
[1] Hanyang Univ, Dept Appl Chem, Ansan 425791, South Korea
关键词
AFM; gold nanoparticles; sandwich DNA hybridization; DNA detection;
D O I
10.1007/s00216-007-1354-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The potential ability of atomic force microscopy (AFM) as a quantitative bioanalysis tool is demonstrated by using gold nanoparticles as a size enhancer in a DNA hybridization reaction. Two sets of probe DNA were functionalized on gold nanoparticles and sandwich hybridization occurred between two probe DNAs and target DNA, resulting in aggregation of the nanoparticles. At high concentrations of target DNA in the range from 100 nM to 10 mu M, the aggregation of gold nanoparticles was determined by monitoring the color change with UV-vis spectroscopy. The absorption spectra broadened after the exposure of DNA-gold nanoparticles to target DNA and a new absorption band at wavelengths > 600 nm was observed. However, no differences were observed in the absorption spectra of the gold nanoparticles at low concentrations of target DNA (10 pM to 10 nM) due to insufficient aggregation. AFM was used as a biosensing tool over this range of target DNA concentrations in order to monitor the aggregation of gold nanoparticles and to quantify the concentration of target DNA. Based on the AFM images, we successfully evaluated particle number and size at low concentrations of target DNA. The calibration curve obtained when mean particle aggregate diameter was plotted against concentration of target DNA showed good linearity over the range 10 pM to 10 nM, the working range for quantitative target DNA analysis. This AFM-based DNA detection technique was three orders of magnitude more sensitive than a DNA detection method based on UV-vis spectroscopy.
引用
收藏
页码:1185 / 1190
页数:6
相关论文
共 26 条
[1]   Controlled and reversible aggregation of biotinylated gold nanoparticles with streptavidin [J].
Aslan, K ;
Luhrs, CC ;
Pérez-Luna, VH .
JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (40) :15631-15639
[2]   Nanogold-plasmon-resonance-based glucose sensing [J].
Aslan, K ;
Lakowicz, JR ;
Geddes, CD .
ANALYTICAL BIOCHEMISTRY, 2004, 330 (01) :145-155
[3]   Gold nanoparticles as a colorimetric sensor for protein conformational changes [J].
Chah, S ;
Hammond, MR ;
Zare, RN .
CHEMISTRY & BIOLOGY, 2005, 12 (03) :323-328
[4]   Formation of aminosilane-functionalized mica for atomic force microscopy imaging of DNA [J].
Crampton, N ;
Bonass, WA ;
Kirkham, J ;
Thomson, NH .
LANGMUIR, 2005, 21 (17) :7884-7891
[5]   Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].
Daniel, MC ;
Astruc, D .
CHEMICAL REVIEWS, 2004, 104 (01) :293-346
[6]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[7]   Designing a novel molecular beacon for surface-immobilized DNA hybridization studies [J].
Fang, XH ;
Liu, XJ ;
Schuster, S ;
Tan, WH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (12) :2921-2922
[8]   DNA and PNA as templates for building nanoassemblies via electrostatic complexation with gold nanoparticles [J].
Gourishankar, A ;
Shukla, S ;
Pasricha, R ;
Sastry, M ;
Ganesh, KN .
CURRENT APPLIED PHYSICS, 2005, 5 (02) :102-107
[9]   PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].
GRABAR, KC ;
FREEMAN, RG ;
HOMMER, MB ;
NATAN, MJ .
ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743
[10]   Escherichia coli RNA polymerase activity observed using atomic force microscopy [J].
Kasas, S ;
Thomson, NH ;
Smith, BL ;
Hansma, HG ;
Zhu, XS ;
Guthold, M ;
Bustamante, C ;
Kool, ET ;
Kashlev, M ;
Hansma, PK .
BIOCHEMISTRY, 1997, 36 (03) :461-468