Selective dehydrogenation of resibufogenin and cinobufagin at 3-OH by Pseudomonas aeruginosa

被引：22

作者：

Zhan, JX

Liu, WH

Guo, HZ

Zhang, YX

Guo, D ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 33卷 / 01期

基金：

中国国家自然科学基金;

关键词：

resibufofenin; cinobufagin; selective dehydrogenation; Pseudomonas aeruginosa;

D O I：

10.1016/S0141-0229(03)00068-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial dehydrogenations of resibufogenin (1) and cinobufagin (2) at 3-OH by Pseudomonas aeruginosa AS 1.860 were investigated. Four products were obtained, which were identified as 3-keto-resibufogenin (3), 3-keto-cinobufagin (4), 3-keto-deacetylcinobufagin (5) and deacetylcinobufagin (6) on the basis of their spectral data. When resibufogenin was added at 22 h and transformed for 2 h, a higher transformation rate was obtained. Compounds 4, 5 and 6 were metabolites of cinobufagin by human intestinal bacteria. (C) 2003 Elsevier Science Inc. All rights reserved.

引用

页码：29 / 32

页数：4

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