Genome Expansion and Gene Loss in Powdery Mildew Fungi Reveal Tradeoffs in Extreme Parasitism

被引:569
作者
Spanu, Pietro D. [1 ]
Abbott, James C. [1 ]
Amselem, Joelle [2 ,16 ]
Burgis, Timothy A. [1 ]
Soanes, Darren M. [3 ]
Stueber, Kurt [4 ]
van Themaat, Emiel Ver Loren [4 ]
Brown, James K. M. [5 ]
Butcher, Sarah A. [1 ]
Gurr, Sarah J. [6 ]
Lebrun, Marc-Henri [16 ]
Ridout, Christopher J. [5 ]
Schulze-Lefert, Paul [4 ]
Talbot, Nicholas J. [3 ]
Ahmadinejad, Nahal [4 ]
Ametz, Christian [1 ]
Barton, Geraint R. [1 ]
Benjdia, Mariam [4 ]
Bidzinski, Przemyslaw [4 ]
Bindschedler, Laurence V. [7 ]
Both, Maike [1 ]
Brewer, Marin T. [8 ]
Cadle-Davidson, Lance [9 ,10 ]
Cadle-Davidson, Molly M. [9 ]
Collemare, Jerome [2 ]
Cramer, Rainer [7 ]
Frenkel, Omer [8 ]
Godfrey, Dale [11 ]
Harriman, James [9 ]
Hoede, Claire [2 ]
King, Brian C. [8 ]
Klages, Sven [12 ]
Kleemann, Jochen [4 ]
Knoll, Daniela [4 ]
Koti, Prasanna S. [4 ]
Kreplak, Jonathan [2 ]
Lopez-Ruiz, Francisco J. [5 ]
Lu, Xunli [4 ]
Maekawa, Takaki [4 ]
Mahanil, Siraprapa [9 ]
Micali, Cristina [4 ]
Milgroom, Michael G. [8 ]
Montana, Giovanni [1 ]
Noir, Sandra [4 ]
O'Connell, Richard J. [4 ]
Oberhaensli, Simone [13 ]
Parlange, Francis [13 ]
Pedersen, Carsten [11 ]
Quesneville, Hadi [2 ]
Reinhardt, Richard [12 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London, England
[2] INRA, Unite Rech Genom Info, F-78026 Versailles, France
[3] Univ Exeter, Sch Biosci, Exeter, Devon, England
[4] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, Cologne, Germany
[5] John Innes Ctr, Dept Dis & Stress Biol, Norwich, Norfolk, England
[6] Univ Oxford, Dept Plant Sci, Oxford, England
[7] Univ Reading, Dept Chem, Reading RG6 2AD, Berks, England
[8] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA
[9] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14853 USA
[10] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA
[11] Univ Copenhagen, Fac Life Sci, Dept Agr & Ecol, Copenhagen, Denmark
[12] Max Planck Inst Mol Genet, Berlin, Germany
[13] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland
[14] Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas UPM INIA, Madrid, Spain
[15] Univ Politecn Madrid, ETSI Agronomos, Madrid, Spain
[16] INRA, Unite BIOGER CPP, Grignon, France
基金
英国生物技术与生命科学研究理事会;
关键词
PLANT-PATHOGENS; VIRULENCE; PROTEINS;
D O I
10.1126/science.1194573
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Powdery mildews are phytopathogens whose growth and reproduction are entirely dependent on living plant cells. The molecular basis of this life-style, obligate biotrophy, remains unknown. We present the genome analysis of barley powdery mildew, Blumeria graminis f.sp. hordei (Blumeria), as well as a comparison with the analysis of two powdery mildews pathogenic on dicotyledonous plants. These genomes display massive retrotransposon proliferation, genome-size expansion, and gene losses. The missing genes encode enzymes of primary and secondary metabolism, carbohydrate-active enzymes, and transporters, probably reflecting their redundancy in an exclusively biotrophic life-style. Among the 248 candidate effectors of pathogenesis identified in the Blumeria genome, very few (less than 10) define a core set conserved in all three mildews, suggesting that most effectors represent species-specific adaptations.
引用
收藏
页码:1543 / 1546
页数:4
相关论文
共 14 条
[1]   The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics [J].
Cantarel, Brandi L. ;
Coutinho, Pedro M. ;
Rancurel, Corinne ;
Bernard, Thomas ;
Lombard, Vincent ;
Henrissat, Bernard .
NUCLEIC ACIDS RESEARCH, 2009, 37 :D233-D238
[2]   Haustorially expressed secreted proteins from flax rust are highly enriched for avirulence elicitors [J].
Catanzariti, AM ;
Dodds, PN ;
Lawrence, GJ ;
Ayliffe, MA ;
Ellis, JG .
PLANT CELL, 2006, 18 (01) :243-256
[3]   Biosynthesis of secondary metabolites in the rice blast fungus Magnaporthe grisea:: the role of hybrid PKS-NRPS in pathogenicity [J].
Collemare, Jerome ;
Billard, Alexis ;
Boehnert, Heidi U. ;
Lebrun, Marc-Henri .
MYCOLOGICAL RESEARCH, 2008, 112 :207-215
[4]  
Debuchy R., 2006, MYCOTA GROWTH DIFFER, P293, DOI DOI 10.1007/3-540-28135-5_15
[5]   The powdery mildews: A review of the world's most familiar (yet poorly known) plant pathogens [J].
Glawe, Dean A. .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2008, 46 :27-51
[7]   Powdery mildew fungal effector candidates share N-terminal Y/F/WxC-motif [J].
Godfrey, Dale ;
Bohlenius, Henrik ;
Pedersen, Carsten ;
Zhang, Ziguo ;
Emmersen, Jeppe ;
Thordal-Christensen, Hans .
BMC GENOMICS, 2010, 11
[8]   DOLLOS LAW AND THE DEATH AND RESURRECTION OF GENES [J].
MARSHALL, CR ;
RAFF, EC ;
RAFF, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (25) :12283-12287
[9]   Aggressive assembly of pyrosequencing reads with mates [J].
Miller, Jason R. ;
Delcher, Arthur L. ;
Koren, Sergey ;
Venter, Eli ;
Walenz, Brian P. ;
Brownley, Anushka ;
Johnson, Justin ;
Li, Kelvin ;
Mobarry, Clark ;
Sutton, Granger .
BIOINFORMATICS, 2008, 24 (24) :2818-2824
[10]   Fungal phytotoxins as mediators of virulence [J].
Moebius, Nadine ;
Hertweck, Christian .
CURRENT OPINION IN PLANT BIOLOGY, 2009, 12 (04) :390-398