Cold sensitivity of recombinant TRPA1 channels

被引:139
作者
Sawada, Yosuke
Hosokawa, Hiroshi
Horia, Aiko
Matsumura, Kiyoshi
Kobayashi, Shigeo [1 ]
机构
[1] Kyoto Univ, Grad Sch Informat, Dept Intelligence Sci & Technol, Div Biol Informat, Kyoto 6068501, Japan
[2] Osaka Inst Technol, Fac Informat Sci & Technol, Osaka 5730196, Japan
基金
日本学术振兴会;
关键词
thermo TRP channel; patch-clamp; Ca imaging; allyl isothiocyanate;
D O I
10.1016/j.brainres.2007.05.047
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
TRPM8 and TRPA1, members of the transient receptor potential (TRP) channel family, are candidates for cooling-activated receptors. It is accepted that TRPM8 responds to moderate cooling, although it is controversial whether TRPA1 responds to deep cooling. Here, using Ca2+ imaging and/or patch-clamp recordings, we examined the thermal sensitivity of primary cultured dorsal root ganglion (DRG) neurons and mouse TRPA1-expressing human embryonic kidney (HEK) 293 cells. In a subset of cultured mouse DRG neurons, deep cooling (5-18 degrees C) and allyl isothiocyanate (AITC, agonist of TRPA1) induced increases in intracellular Ca 2, level. Most AITC-sensitive (TRPA1-expressing) neurons responded to deep cooling. In TRPA1-expressing HEK293 cells, deep cooling and AITC-induced Ca2+ responses and whole-cell currents. in inside-out patches excised from TRPA1-expressing HEK293 cells, deep cooling, and AITC activated the same channels, which were inhibited by camphor (antagonist for TRPA1). When temperature was decreased below 18 degrees C, unit conductance of the channel decreased but open probability of it increased. Deep cooling-induced increase of the open probability of TRPA1 may underlie the increase in whole-cell currents induced by deep cooling. It is concluded that TRPA1 is a deep cooling-activated channel, which supports the previous findings that TRPA1 responds to deep cooling. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:39 / 46
页数:8
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