Evidence that biliverdin-IX beta reductase and flavin reductase are identical

被引：39

作者：

Shalloe, F ^{[1
]}

Elliott, G ^{[1
]}

Ennis, O ^{[1
]}

Mantle, TJ ^{[1
]}

机构：

[1] TRINITY COLL DUBLIN,DEPT BIOCHEM,DUBLIN 2,IRELAND

来源：

BIOCHEMICAL JOURNAL | 1996年 / 316卷

关键词：

D O I：

10.1042/bj3160385

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A search of the database shows that human biliverdin-IX beta reductase and flavin reductase are identical. We have isolated flavin reductase from bovine erythrocytes and show that the activity co-elutes with biliverdin-IX beta reductase. Preparations of the enzyme that are electrophoretically homogeneous exhibit both flavin reductase and biliverdin-IX beta reductase activities; however, they are not capable of catalysing the reduction of biliverdin-IX alpha. Although there is little obvious sequence identity between biliverdin-IX alpha reductase (BVR-A) and biliverdin-IX beta reductase (BVR-B), they do show weak immunological cross-reactivity. Both enzymes bind to 2',5'-ADP-Sepharose.

引用

页码：385 / 387

页数：3