Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase

被引：56

作者：

Olano, C

Wilkinson, B

Moss, SJ

Braña, AF

Méndez, C

Leadlay, PF

Salas, JA ^{[1
]}

机构：

[1] Univ Oviedo, Dept Biol Func, E-33006 Oviedo, Spain

[2] Univ Oviedo, IUOPA, E-33006 Oviedo, Spain

[3] Biot Technol Ltd, Cambridge CB3 0DJ, England

[4] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

CHEMICAL COMMUNICATIONS | 2003年 / 22期

关键词：

D O I：

10.1039/b310648a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Functional evidence for programmed loss of co-linearity on the borrelidin modular polyketide synthase (PKS) is presented.

引用

页码：2780 / 2782

页数：3

共 21 条

[1] The hidden steps of domain skipping: Macrolactone ring size determination in the pikromycin modular polyketide synthase [J].

Beck, BJ ;

Yoon, YJ ;

Reynolds, KA ;

Sherman, DH .

CHEMISTRY & BIOLOGY, 2002, 9 (05) :575-583

[2]

BERGER J, 1949, ARCH BIOCHEM, V22, P476

[3] Metabolic engineering - a genetic toolbox for small molecule organic synthesis [J].

Burkart, MD .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (01) :1-4

[4]

Funahashi Y, 1999, ONCOL RES, V11, P319

[5] The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase [J].

Gaitatzis, N ;

Silakowski, B ;

Kunze, B ;

Nordsiek, G ;

Blöcker, H ;

Höfle, G ;

Müller, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (15) :13082-13090

[6] Role of linkers in communication between protein modules [J].

Gokhale, RS ;

Khosla, C .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (01) :22-27

[7] New natural epothilones from Sorangium cellulosum, strains So ce90/B2 and So ce90/D13:: Isolation, structure elucidation, and SAR studies [J].

Hardt, IH ;

Steinmetz, H ;

Gerth, K ;

Sasse, F ;

Reichenbach, H ;

Höfle, G .

JOURNAL OF NATURAL PRODUCTS, 2001, 64 (07) :847-856

[8] Genetic contributions to understanding polyketide synthases [J].

Hopwood, DA .

CHEMICAL REVIEWS, 1997, 97 (07) :2465-2497

[9] Natural product biosynthesis: A new interface between enzymology and medicine [J].

Khosla, C .

JOURNAL OF ORGANIC CHEMISTRY, 2000, 65 (24) :8127-8133

[10] The crystal structure of β-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54 Å resolution and its relationship to other condensing enzymes [J].

Moche, M ;

Dehesh, K ;

Edwards, P ;

Lindqvist, Y .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (03) :491-503

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