Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients

被引：17

作者：

GallezHawkins, GM

Tegtmeier, BR

terVeer, A

Niland, JC

Forman, SJ

Zaia, JA

机构：

[1] CITY HOPE NATL MED CTR,DEPT PEDIAT,DUARTE,CA 91010

[2] CITY HOPE NATL MED CTR,DEPT INFECT DIS CLIN PATHOL,DUARTE,CA 91010

[3] CITY HOPE NATL MED CTR,DEPT BIOSTAT,DUARTE,CA 91010

[4] CITY HOPE NATL MED CTR,DEPT HEMATOL BONE MARROW TRANSPLANTAT,DUARTE,CA 91010

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 03期

关键词：

D O I：

10.1128/JCM.35.3.788-790.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.

引用

页码：788 / 790

页数：3

共 24 条

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