Ferrocene-conjugated m-phenylenediamine conducting polymer-incorporated peroxidase biosensors

被引：38

作者：

Mulchandani, A ^{[1
]}

Pan, ST ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Environm Chem & Engn, Riverside, CA 92521 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 267卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1006/abio.1998.2983

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development and characteristics of a reagentless amperometric biosensor employing horseradish peroxidase incorporated in an electrochemically deposited ferrocene-modified phenylenediamine film on a glassy carbon electrode is reported. The horseradish peroxidase/poly(m-aminoanilinomethylferrocene)- modified glassy carbon electrode reagentless biosensor measured hydrogen peroxide and other organic peroxides in both aqueous and organic medium by reduction at a low applied potential of -0.05 V (vs Ag/AgCl) without interference from molecular oxygen. When modified with glucose oxidase, the new bienzyme electrode measured glucose sensitively and selectively, demonstrating the suitability of the above peroxide biosensor for other oxidoreductase enzyme-based biosensors. (C) 1999Academic Press.

引用

页码：141 / 147

页数：7

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