A CARRAGEENAN HYDROGEL STABILIZED COLLOIDAL GOLD MULTIENZYME BIOSENSOR ELECTRODE UTILIZING IMMOBILIZED HORSERADISH-PEROXIDASE AND CHOLESTEROL OXIDASE CHOLESTEROL ESTERASE TO DETECT CHOLESTEROL IN SERUM AND WHOLE-BLOOD

被引：74

作者：

CRUBLISS, AL ^{[1
]}

STONEHUERNER, JG ^{[1
]}

HENKENS, RW ^{[1
]}

ZHAO, J ^{[1
]}

ODALY, JP ^{[1
]}

机构：

[1] ENZYME TECHNOL RES GRP INC, DURHAM, NC 27701 USA

来源：

BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 06期

关键词：

BIOSENSOR; ELECTRODE; HYDROGEL; CHOLESTEROL; HORSERADISH PEROXIDASE; CHOLESTEROL OXIDASE; COLLOIDAL GOLD;

D O I：

10.1016/0956-5663(93)85014-F

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The preparation of two immobilized enzyme electrodes is described. One electrode contains horseradish peroxidase adsorbed to colloidal gold and deposited on a glassy carbon electrode along with cholesterol oxidase entrapped in a carrageenan hydrogel. The second electrode also includes cholesterol esterase entrapped in the carrageenan. The incorporation of ferrocene or ferrocenecarboxylic acid mediator is brought about by either evaporation on the glassy carbon electrode or, in the latter case, entrapment in the carrageenan hydrogel. Amperometric signal generation results from the HRP catalyzed turnover of H2O2, a secondary product of the cholesterol oxidase catalyzed oxidation of cholesterol. Use of these enzyme electrodes makes cholesterol detection possible in human serum, low density lipoprotein, and whole blood.

引用

页码：331 / 337

页数：7

共 18 条

[1]

ALLAIN CC, 1974, CLIN CHEM, V20, P470

[2] THE DETERMINATION OF TOTAL SERUM-CHOLESTEROL BY FLOW-INJECTION ANALYSIS WITH AMPEROMETRIC DETECTION [J].