Isolation of oligogalacturonic acids up to DP 20 by preparative high-performance anion-exchange chromatography and pulsed amperometric detection

被引：22

作者：

Hotchkiss, AT ^{[1
]}

Lecrinier, SL ^{[1
]}

Hicks, KB ^{[1
]}

机构：

[1] USDA, ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

CARBOHYDRATE RESEARCH | 2001年 / 334卷 / 02期

关键词：

oligogalacturonic acid; degree of polymerization; DP; 20; high-performance anion-exchange chromatography; pulsed amperometric detection; preparative HPAEC-PAD; CarboPac PA1 column;

D O I：

10.1016/S0008-6215(01)00170-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Milligram quantities of oligogalacturonic acids up to a degree of polymerization (DP) of 20 were purified by high-performance anion-exchange chromatography utilizing a preparative-scale (21-mm i.d.) CarboPac PA1 column and a nonlinear potassium acetate (pH 7.5) gradient. Detection was accomplished by pulsed amperometry without post-column addition of hydroxide. Pulsed amperometry at near-neutral pH is an excellent detection method for preparative separations of carbohydrates because it avoids base-catalyzed degradation reactions that can occur at high pH. This method was simpler. faster, had higher sample loading capacity and allowed for the isolation of higher DP oligogalacturonic acids than other methods reported previously. With this improved method, multi-milligram quantities of valuable oligogalacturonic acids (up to DP 20) can be readily isolated. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：135 / 140

页数：6

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