Molecular cloning and functional characterization of rat Δ-6 fatty acid desaturase

被引：112

作者：

Aki, T ^{[1
]}

Shimada, Y ^{[1
]}

Inagaki, K ^{[1
]}

Higashimoto, H ^{[1
]}

Kawamoto, S ^{[1
]}

Shigeta, S ^{[1
]}

Ono, K ^{[1
]}

Suzuki, O ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Mol Biotechnol, Higashihiroshima 7398527, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.0235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian cDNA fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (EST) sequence informations. These fragments were subsequently used to screen a rat liver cDNA library, yielding a 1573-bp clone. Expression of DNA fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence of exogenous linoleic acid. In this system, the addition of alpha-linolenic acid also resulted in the accumulation of its Delta-6 desaturated product whereas dihomo-gamma-linolenic acid failed to be a substrate. These results indicate that the protein encoded by the rat cDNA is Delta-6 fatty acid desaturase, and the first 17 amino acids corresponding to the coding region 97-147 of the clone are not required to function in yeast. (C) 1999 Academic Press.

引用

页码：575 / 579

页数：5

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