High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling

被引：388

作者：

Brar, Gloria A. ^{[1
,2
]}

Yassour, Moran ^{[3
,4
,5
]}

Friedman, Nir ^{[5
,6
]}

Regev, Aviv ^{[3
,4
]}

Ingolia, Nicholas T. ^{[1
,2
]}

Weissman, Jonathan S. ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Cellular & Mol Pharmacol, San Francisco, CA 94158 USA

[2] Calif Inst Quantitat Biosci, San Francisco, CA 94158 USA

[3] Massachusetts Inst Technol & Harvard, Broad Inst, Cambridge, MA 02142 USA

[4] MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA 02139 USA

[5] Hebrew Univ Jerusalem, Sch Engn & Comp Sci, IL-91904 Jerusalem, Israel

[6] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, IL-91904 Jerusalem, Israel

来源：

SCIENCE | 2012年 / 335卷 / 6068期

关键词：

UNFOLDED PROTEIN-RESPONSE; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; ENDOPLASMIC-RETICULUM; SYNAPTONEMAL COMPLEX; TRANSCRIPTION FACTOR; BUDDING YEAST; MEIOSIS; GAMETOGENESIS; CHROMOSOMES;

D O I：

10.1126/science.1215110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5' leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.

引用

页码：552 / 557

页数：6

共 40 条

[1] 5′UTR Variants of Ribosomal Protein S19 Transcript Determine Translational Efficiency: Implications for Diamond-Blackfan Anemia and Tissue Variability [J].