Column liquid chromatography determination of vitamins A and E in powdered milk and local flour: a validation procedure

被引：20

作者：

Ake, M

Fabre, H

Malan, AK

Mandrou, B

机构：

[1] Fac Pharm Montpellier, Chim Analyt Lab, F-34060 Montpellier 2, France

[2] Fac Pharm, Chim Analyt Lab, Abidjan 01, Cote Ivoire

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 826卷 / 02期

关键词：

validation; vitamins; food analysis;

D O I：

10.1016/S0021-9673(98)00747-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 189

页数：7

共 14 条

[1] TOCOPHEROL MICRO-EXTRACTION METHOD WITH APPLICATION TO QUANTITATIVE-ANALYSIS OF LIPOPHILIC NUTRIENTS [J].