Methamphetamine causes widespread apoptosis in the mouse brain: evidence from using an improved TUNEL histochemical method

被引：168

作者：

Deng, XL ^{[1
]}

Wang, Y ^{[1
]}

Chou, J ^{[1
]}

Cadet, JL ^{[1
]}

机构：

[1] NIDA, NIH, IRP, Baltimore, MD 21224 USA

来源：

MOLECULAR BRAIN RESEARCH | 2001年 / 93卷 / 01期

关键词：

methamphetamine; TUNEL; apoptosis; ischemia;

D O I：

10.1016/S0169-328X(01)00184-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Terminal deoxynucleotidyl transferase (TdT)-mediated dNTP nick end labeling (TUNEL) histochemistry is a sensitive method to expose DNA strand breaks in apoptotic cells. but it is difficult to conduct on slide-mounted sections. By using a 80 degreesC/0.5% Triton X-100 pretreatment, we have developed a TUNEL histochemical approach with high specificity and sensitivity using sections from ischemic rat brains. Thereafter. methamphetamine (METH)-induced neuronal death was investigated in mice brains. The results showed that a single injection of 40 mg/kg METH caused neuronal death in several brain areas including the striatum, cortex (frontal, parietal, and piriform), indusium griseum, medial habenular nucleus. and hippocampus. These results further confirmed the presence of METH-induced deleterious effects in nondopaminergic neurons. The significance of these findings is also discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：64 / 69

页数：6

共 37 条

[31]

Shaw KP, 1999, J FORENSIC SCI, V44, P27

[32]

Stähelin BJ, 1998, J CLIN PATHOL-MOL PA, V51, P204

[33] EFFECT OF REPEATED METHAMPHETAMINE ADMINISTRATIONS ON DOPAMINE AND GLUTAMATE EFFLUX IN RAT PREFRONTAL CORTEX [J].