Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes

被引:251
作者
Wagner, M [1 ]
Horn, M [1 ]
Daims, H [1 ]
机构
[1] Univ Vienna, Inst Ecol & Conservat Biol, Dept Microbial Ecol, A-1090 Vienna, Austria
关键词
D O I
10.1016/S1369-5274(03)00054-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological improvements in fluorescence in situ hybridisation, as well as in the combination of this method with other techniques for inferring functional traits of microorganisms within their environment.
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页码:302 / 309
页数:8
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