The identification of microorganisms by fluorescence in situ hybridisation

被引:258
作者
Amann, R [1 ]
Fuchs, BM [1 ]
Behrens, S [1 ]
机构
[1] Max Planck Inst Marine Microbiol, Mol Ecol Grp, D-28359 Bremen, Germany
关键词
D O I
10.1016/S0958-1669(00)00204-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence in situ hybridisation (FISH) with rRNA-targeted oligonucleotide probes facilitates the rapid and specific identification of individual microbial cells in their natural environments. Over the past year there have been a number of methodological developments in this area and new applications of FISH in microbial ecology and biotechnology have been reported.
引用
收藏
页码:231 / 236
页数:6
相关论文
共 73 条
[1]   In situ methods for assessment of microorganisms and their activities [J].
Amann, R ;
Kuhl, M .
CURRENT OPINION IN MICROBIOLOGY, 1998, 1 (03) :352-358
[2]   Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology [J].
Amann, R ;
Ludwig, W .
FEMS MICROBIOLOGY REVIEWS, 2000, 24 (05) :555-565
[3]  
Amann R. I., MOL MICROBIAL ECOLOG, P1
[4]   FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].
AMANN, RI ;
KRUMHOLZ, L ;
STAHL, DA .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :762-770
[5]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[6]   Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds [J].
Antón, J ;
Llobet-Brossa, E ;
Rodríguez-Valera, F ;
Amann, R .
ENVIRONMENTAL MICROBIOLOGY, 1999, 1 (06) :517-523
[7]   Extremely halophilic Bacteria in crystallizer ponds from solar salterns [J].
Antón, J ;
Rosselló-Mora, R ;
Rodríguez-Valera, F ;
Amann, R .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (07) :3052-3057
[8]   Molecular and ecological evidence for species specificity and coevolution in a group of marine algal-bacterial symbioses [J].
Ashen, JB ;
Goff, LJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (07) :3024-3030
[9]   Characterization of the microbial community of lotic organic aggregates ('river snow') in the Elbe River of Germany by cultivation and molecular methods [J].
Böckelmann, U ;
Manz, W ;
Neu, TR ;
Szewzyk, U .
FEMS MICROBIOLOGY ECOLOGY, 2000, 33 (02) :157-170
[10]   A marine microbial consortium apparently mediating anaerobic oxidation of methane [J].
Boetius, A ;
Ravenschlag, K ;
Schubert, CJ ;
Rickert, D ;
Widdel, F ;
Gieseke, A ;
Amann, R ;
Jorgensen, BB ;
Witte, U ;
Pfannkuche, O .
NATURE, 2000, 407 (6804) :623-626