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Detection of the enterotoxins A, B, and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds

被引：46
作者：
da Silva, ER
do Carmo, LS
da Silva, N
机构：
[1] Univ Fed Minas Gerais, Sch Vet, Lab Mastitis Control, Belo Horizonte, MG, Brazil
[2] Fundacao Ezequiel Dias, Lab Staphylococcal Enterotoxins, Belo Horizonte, MG, Brazil
来源：
VETERINARY MICROBIOLOGY | 2005年 / 106卷 / 1-2期
关键词：
goat mastitis; bovine mastitis; Staphylococcal enterotoxins;
D O I：
10.1016/j.vetmic.2004.12.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To determine the distribution of genes that encode enterotoxins A, B and C, 36 strains of Staphylococcus aureus isolated from goat mastitis and 64 isolated from bovine mastitis were analyzed by Multiplex PCR. Of the total strains studied, 37 (37%) were detected to have some of the SEs genes. From the bovine mastitis strains, 4 (6.3%) co-amplified the sea and seb genes and 2 (3.1%) were positive for the sec gene. From the goat mastitis strains, 31 (86%) tested positive to the Multiplex, and the sec gene was detected in all of them. The production of SE was, detected in all strains harboring the corresponding gene. The results demonstrated that S. aureus isolated from goat mastitis had a higher enterotoxigenic potential than those isolated from bovine mastitis. Additionally, the presence of the sec gene in the majority of goat mastitis strains suggests a possible involvement of SEC in goat mastitis pathogenesis. (c) 2004 Elsevier B.V. All rights reserved.
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页码：103 / 107
页数：5
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