Very fast two-dimensional NMR spectroscopy for real-time investigation of dynamic events in proteins on the time scale of seconds

被引：536

作者：

Schanda, P ^{[1
]}

Brutscher, B ^{[1
]}

机构：

[1] UJF, CEA, CNRS, Inst Biol Struct Jean Pierre Ebel, F-38027 Grenoble, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 22期

关键词：

D O I：

10.1021/ja051306e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate for different protein samples that 2D 1H-15N correlation NMR spectra can be recorded in a few seconds of acquisition time using a new band-selective optimized flip-angle short-transient heteronuclear multiple quantum coherence experiment. This has enabled us to measure fast hydrogen-deuterium exchange rate constants along the backbone of a small globular protein fragment by real-time 2D NMR. Copyright © 2005 American Chemical Society.

引用

页码：8014 / 8015

页数：2

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