Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

被引：41

作者：

Izumiya, Hidemasa ^{[1
]}

Matsumoto, Kazutoshi ^{[2
]}

Yahiro, Shunsuke ^{[2
]}

Lee, Jiyoung ^{[1
]}

Morita, Masatomo ^{[1
]}

Yamamoto, Shouji ^{[1
]}

Arakawa, Eiji ^{[1
]}

Ohnishi, Makoto ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Bacteriol 1, Shinjuku Ku, Tokyo 1628640, Japan

[2] Kumamoto Prefectural Inst Publ Hlth & Environm Sc, Uto City, Kumamoto 8690425, Japan

来源：

MOLECULAR AND CELLULAR PROBES | 2011年 / 25卷 / 04期

关键词：

atpA; Multiplex PCR; V cholerae; V parahaemolyticus; V vulnificus; SP NOV; GENE;

D O I：

10.1016/j.mcp.2011.04.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：174 / 176

页数：3

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