学术探索
学术期刊
新闻热点
数据分析
智能评审

POLYMERASE CHAIN-REACTION IDENTIFICATION OF VIBRIO-VULNIFICUS IN ARTIFICIALLY CONTAMINATED OYSTERS

被引：141
作者：
HILL, WE
KEASLER, SP
TRUCKSESS, MW
FENG, P
KAYSNER, CA
LAMPEL, KA
机构：
[1] US FDA,DIV CONTAMINANTS CHEM,WASHINGTON,DC 20204
[2] US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204
[3] US FDA,SEAFOOD PROD RES CTR,BOTHELL,WA 98021
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 03期
关键词：
D O I：
10.1128/AEM.57.3.707-711.1991
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
DNAs extracted from Vibrio vulnificus seeded into oyster homogenates were evaluated as templates for the polymerase chain reaction. Several extraction procedures were examined, and it was determined that DNA recovered from cells lysed by guanidine isothiocyanate, extracted with chloroform, and precipitated with ethanol was most suitable for use as a polymerase chain reaction template. The region targeted was a 519-bp portion of the cytotoxin-hemolysin gene of V. vulnificus. This region was amplified only when DNA from this species was present in the homogenate. V. vulnificus seeded into oyster homogenates at an initial level of 10(2) CFU/g of oyster meat was consistently observed after 24 h of incubation in alkaline peptone water.
引用
收藏
页码：707 / 711
页数：5
相关论文
共 24 条
[1]   DETECTION OF COLIFORM BACTERIA IN WATER BY POLYMERASE CHAIN-REACTION AND GENE PROBES [J].
BEJ, AK ;
STEFFAN, RJ ;
DICESARE, J ;
HAFF, L ;
ATLAS, RM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (02) :307-314
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]   GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].
BLIN, N ;
STAFFORD, DW .
NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308
[4]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[5]  
CURIALE MS, 1990, J ASSOC OFF ANA CHEM, V73, P248
[6]   DETECTION AND IDENTIFICATION OF ESCHERICHIA-COLI PRODUCING HEAT-LABILE ENTEROTOXIN TYPE-I BY ENZYMATIC AMPLIFICATION OF A SPECIFIC DNA FRAGMENT [J].
FURRER, B ;
CANDRIAN, U ;
LUTHY, J .
LETTERS IN APPLIED MICROBIOLOGY, 1990, 10 (01) :31-34
[7]   PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR CYTOLYSIN PRODUCED BY VIBRIO-VULNIFICUS [J].
GRAY, LD ;
KREGER, AS .
INFECTION AND IMMUNITY, 1985, 48 (01) :62-72
[8]   ENUMERATION OF VIRULENT YERSINIA-ENTEROCOLITICA COLONIES BY DNA COLONY HYBRIDIZATION USING ALKALINE TREATMENT AND PAPER FILTERS [J].
JAGOW, JA ;
HILL, WE .
MOLECULAR AND CELLULAR PROBES, 1988, 2 (03) :189-195
[9]   GASTROENTERITIS IN PATIENTS WITH STOOL ISOLATES OF VIBRIO-VULNIFICUS [J].
JOHNSTON, JM ;
BECKER, SF ;
MCFARLAND, LM .
AMERICAN JOURNAL OF MEDICINE, 1986, 80 (02) :336-338
[10]   VIRULENT-STRAINS OF VIBRIO-VULNIFICUS ISOLATED FROM ESTUARIES OF THE UNITED-STATES WEST-COAST [J].
KAYSNER, CA ;
ABEYTA, C ;
WEKELL, MM ;
DEPAOLA, A ;
STOTT, RF ;
LEITCH, JM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (06) :1349-1351
123