POLYMERASE CHAIN-REACTION IDENTIFICATION OF VIBRIO-VULNIFICUS IN ARTIFICIALLY CONTAMINATED OYSTERS

被引：141

作者：

HILL, WE

KEASLER, SP

TRUCKSESS, MW

FENG, P

KAYSNER, CA

LAMPEL, KA

机构：

[1] US FDA,DIV CONTAMINANTS CHEM,WASHINGTON,DC 20204

[2] US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204

[3] US FDA,SEAFOOD PROD RES CTR,BOTHELL,WA 98021

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 03期

关键词：

D O I：

10.1128/AEM.57.3.707-711.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNAs extracted from Vibrio vulnificus seeded into oyster homogenates were evaluated as templates for the polymerase chain reaction. Several extraction procedures were examined, and it was determined that DNA recovered from cells lysed by guanidine isothiocyanate, extracted with chloroform, and precipitated with ethanol was most suitable for use as a polymerase chain reaction template. The region targeted was a 519-bp portion of the cytotoxin-hemolysin gene of V. vulnificus. This region was amplified only when DNA from this species was present in the homogenate. V. vulnificus seeded into oyster homogenates at an initial level of 10(2) CFU/g of oyster meat was consistently observed after 24 h of incubation in alkaline peptone water.

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收藏

页码：707 / 711

页数：5

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