Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation

被引:339
作者
Allen, Andrew E. [2 ]
LaRoche, Julie [1 ]
Maheswari, Uma [2 ]
Lommer, Markus [1 ]
Schauer, Nicolas [3 ]
Lopez, Pascal J. [2 ]
Finazzi, Giovanni [4 ]
Fernie, Alisclair R. [3 ]
Bowler, Chris [2 ,5 ]
机构
[1] Leibniz Inst Meereswissensch, D-24105 Kiel, Germany
[2] Ecole Normale Super, Ctr Natl Rech Sci Unite Mixte Rech 8186, Dept Biol, F-75005 Paris, France
[3] Max Planck Inst Mol Physiol, D-14476 Potsdam, Germany
[4] Univ Paris 06, Ctr Natl Rech Sci Unite Mixte Rech 7141, Inst Biol Physicochim, F-75005 Paris, France
[5] Stn Zool, I-80121 Naples, Italy
关键词
genome; metabalomics; photosynthesis; transcriptomics; nutrients;
D O I
10.1073/pnas.0711370105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Marine primary productivity is iron (Fe)-limited in vast regions of the contemporary oceans, most notably the high nutrient low chlorophyll (HNLC) regions. Diatoms often form large blooms upon the relief of Fe limitation in HNLC regions despite their prebloom low cell density. Although Fe plays an important role in controlling diatom distribution, the mechanisms of Fe uptake and adaptation to low iron availability are largely unknown. Through a combination of nontargeted transcriptomic and metabolomic approaches, we have explored the biochemical strategies preferred by Phaeodactylum tricornutum at growth-limiting levels of dissolved Fe. Processes carried out by components rich in Fe, such as photosynthesis, mitochondrial electron transport, and nitrate assimilation, were down-regulated. Our results show that this retrenchment is compensated by nitrogen (N) and carbon (C) reallocation from protein and carbohydrate degradation, adaptations to chlorophyll biosynthesis and pigment metabolism, removal of excess electrons by mitochondrial alternative oxidase (AOX) and non-photochemical quenching (NPQ), and augmented Fe-independent oxidative stress responses. Iron limitation leads to the elevated expression of at least three gene clusters absent from the Thalassiosira pseudonana genome that encode for components of iron capture and uptake mechanisms.
引用
收藏
页码:10438 / 10443
页数:6
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