Effects of homocysteine on the binding of extracellular-superoxide dismutase to the endothelial cell surface

被引：96

作者：

Yamamoto, M ^{[1
]}

Hara, H ^{[1
]}

Adachi, T ^{[1
]}

机构：

[1] Gifu Pharmaceut Univ, Lab Clin Pharmaceut, Gifu 5028585, Japan

来源：

FEBS LETTERS | 2000年 / 486卷 / 02期

关键词：

homocysteine; extracellular-superoxide dismutase; endothelial cell; heparan sulfate proteoglycan; atherosclerosis;

D O I：

10.1016/S0014-5793(00)02260-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homocysteine is known to be a risk factor for several vascular diseases. Previously, we found a significant association between plasma homocysteine and plasma extracellular-superoxide dismutase (EC-SOD) levels. The binding of EC-SOD to human and bovine aortic endothelial cell cultures showed significant decreases after incubation with 10 muM homocysteine, whereas the expression of EC-SOD in fibroblast cell cultures was inhibited with a high concentration (1 mM) of homocysteine. Furthermore, binding of EC-SOD to heparin immobilized on plates was decreased with homocysteine. These observations suggested that homocysteine decreases the binding of EC-SOD to vascular endothelial cell surfaces by degradation of endothelial heparan sulfate proteoglycan, which results in a loss of the ability to protect endothelial cell surfaces from oxidative stress. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：159 / 162

页数：4

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