Purification and some properties of β-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404

被引：34

作者：

Yoshikawa, Jun ^{[1
]}

Amachi, Seigo ^{[1
]}

Shinoyama, Hirofumi ^{[1
]}

Fujii, Takaaki ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Sci & Technol, Dept Adv Bioresources Sci, Matsudo, Chiba 2718510, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2007年 / 103卷 / 05期

关键词：

beta-fructofuranosidase; Aureobasidium pullulans; fructooligosaccharide; transfructosylation;

D O I：

10.1263/jbb.103.491

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

beta-Fructofuranosidase I (FFase I) formed by Aureobasidium pullulans DSM 2404 was purified. The enzyme had a molecular weight of about 430 kDa, was not affected by various metal ions and showed high transfructosylating activity. The yield of fructooligosaccharides production using purified FFase I was 62%.

引用

页码：491 / 493

页数：3

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