Embryonic brain precursors transplanted into kainate lesioned rat spinal cord

被引:12
作者
Magnuson, DSK [1 ]
Zhang, YP [1 ]
Cao, QL [1 ]
Han, YC [1 ]
Burke, DA [1 ]
Whittemore, SR [1 ]
机构
[1] Univ Louisville, Sch Med, Dept Neurol Surg, Louisville, KY 40202 USA
关键词
kainic acid; neural precursor cells; neural stem cells; spinal cord injury; transplantation;
D O I
10.1097/00001756-200104170-00030
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Embryonic day 14 rat cerebral cortex-derived precursors were expanded with FGF2 and labeled with BrdU prior to being transplanted into the kainic acid-lesioned adult rat spinal cord. While these precursors give rise to cells with neuronal, astrocytic and oligodendroglial phenotypes in vitro, they remained largely undifferentiated up to 12 weeks in vivo. Numerous BrdU-labeled cells were found in injured gray matter, and also lining the dilated central canal that: sometimes accompanies these lesions. BrdU-labeled cells never coexpressed Map2ab,rarely co-expressed GFAP but often coexpressed nestin, even after 12 weeks in vivo. These observations suggest that the environment of the kainic acid-injured spinal cord is not hostile to transplanted embryonic cerebral cortex-derived precursors, but also is not conducive to their neuronal differentation. NeuroReport 12:1015-1019 (C) 2001 Lippincott Williams & Wilkins.
引用
收藏
页码:1015 / 1019
页数:5
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