Determination of F1 hybrid seed purity in pepper using PCR-based markers

被引：46

作者：

Ballester, J ^{[1
]}

de Vicente, MC ^{[1
]}

机构：

[1] IRTA, Dept Genet Vegetal, Cabrils 08348, Spain

来源：

EUPHYTICA | 1998年 / 103卷 / 02期

关键词：

Capsicum annuum; hybrid purity; RAPDs; SPARs;

D O I：

10.1023/A:1018372523343

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We present the potential use of two kinds of PCR-based markers, RAPDs (Random Amplified Polymorphic DNA) and SPARs (Single Primer Amplification Reactions), as tools for hybrid seed purity determination. Five Fl pepper hybrids (Capsicum annuum L.) and their parents were analyzed with 100 10-mer primers and 10 nucleotide repeat primers. We found at least one useful marker for testing the purity of all hybrids studied. Despite their dominant inheritance these markers could be an efficient implement in the process of quality testing of hybrid seeds.

引用

收藏

页码：223 / 226

页数：4

相关论文

共 14 条

[1] APPLICATION OF ISOZYME ELECTROPHORESIS FOR PURITY TESTING AND CULTIVAR IDENTIFICATION OF F1 HYBRIDS OF BRASSICA-OLERACEA [J].

ARUS, P ;

SHIELDS, CR ;

ORTON, TJ .

EUPHYTICA, 1985, 34 (03) :651-657

[2] DESIGNING THE OPTIMAL OLIGO [J].

BINNIE, C .

TRENDS IN BIOTECHNOLOGY, 1991, 9 (05) :145-146

[3] MICROPREP PROTOCOL FOR EXTRACTION OF DNA FROM TOMATO AND OTHER HERBACEOUS PLANTS [J].

FULTON, TM ;

CHUNWONGSE, J ;

TANKSLEY, SD .

PLANT MOLECULAR BIOLOGY REPORTER, 1995, 13 (03) :207-209

[4] AMPLIFICATION OF DNA MARKERS FROM EVOLUTIONARILY DIVERSE GENOMES USING SINGLE PRIMERS OF SIMPLE-SEQUENCE REPEATS [J].

GUPTA, M ;

CHYI, YS ;

ROMEROSEVERSON, J ;

OWEN, JL .

THEORETICAL AND APPLIED GENETICS, 1994, 89 (7-8) :998-1006

[5]

HeiserJr CB, 1979, EVOLUTION CROP PLANT, P265

[6] NUCLEAR RFLP BETWEEN PEPPER CULTIVARS (CAPSICUM-ANNUUM-L) [J].

LEFEBVRE, V ;

PALLOIX, A ;

RIVES, M .

EUPHYTICA, 1993, 71 (03) :189-199

[7]

LIVNEH O, 1990, SEED SCI TECHNOL, V18, P209

[8] RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS ARE USEFUL FOR PURITY DETERMINATION OF TOMATO HYBRIDS [J].

PARAN, I ;

HOROWITZ, M ;

ZAMIR, D ;

WOLF, S .

HORTSCIENCE, 1995, 30 (02) :377-377

[9] A SURVEY OF DNA POLYMORPHISM WITHIN THE GENUS CAPSICUM AND THE FINGERPRINTING OF PEPPER CULTIVARS [J].

PRINCE, JP ;

LACKNEY, VK ;

ANGELES, C ;

BLAUTH, JR ;

KYLE, MM .

GENOME, 1995, 38 (02) :224-231

[10] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491