Determination of F1 hybrid seed purity in pepper using PCR-based markers

被引：46

作者：

Ballester, J ^{[1
]}

de Vicente, MC ^{[1
]}

机构：

[1] IRTA, Dept Genet Vegetal, Cabrils 08348, Spain

来源：

EUPHYTICA | 1998年 / 103卷 / 02期

关键词：

Capsicum annuum; hybrid purity; RAPDs; SPARs;

D O I：

10.1023/A:1018372523343

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We present the potential use of two kinds of PCR-based markers, RAPDs (Random Amplified Polymorphic DNA) and SPARs (Single Primer Amplification Reactions), as tools for hybrid seed purity determination. Five Fl pepper hybrids (Capsicum annuum L.) and their parents were analyzed with 100 10-mer primers and 10 nucleotide repeat primers. We found at least one useful marker for testing the purity of all hybrids studied. Despite their dominant inheritance these markers could be an efficient implement in the process of quality testing of hybrid seeds.

引用

页码：223 / 226

页数：4