Salt stress proteins identified by a functional approach in yeast

被引:15
作者
Serrano, R [1 ]
Gaxiola, R
Ríos, G
Forment, J
Vicente, O
Ros, R
机构
[1] Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, CSIC, E-46022 Valencia, Spain
[2] Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA
来源
MONATSHEFTE FUR CHEMIE | 2003年 / 134卷 / 11期
关键词
Arabidopsis; genomics; salt stress; sugar beet; yeast;
D O I
10.1007/s00706-002-0606-4
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have performed functional genomics of salt stress by overexpression of gene libraries in yeast and selection for salt tolerance. Thirty halotolerance genes were isolated from yeast, Arabidopsis, and sugar beet. The results indicate that Na+ transport (uptake, efflux, and compartmentation), sulfate activation, RNA processing, and protein synthesis are crucial for salt tolerance.
引用
收藏
页码:1445 / 1464
页数:20
相关论文
共 130 条
[61]   Tcn1p/Crz1p a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae [J].
Matheos, DP ;
Kingsbury, TJ ;
Ahsan, US ;
Cunningham, KW .
GENES & DEVELOPMENT, 1997, 11 (24) :3445-3458
[62]   SALT-INDUCIBLE BETAINE ALDEHYDE DEHYDROGENASE FROM SUGAR-BEET - CDNA CLONING AND EXPRESSION [J].
MCCUE, KF ;
HANSON, AD .
PLANT MOLECULAR BIOLOGY, 1992, 18 (01) :1-11
[63]   Yeast putative transcription factors involved in salt tolerance [J].
Mendizabal, I ;
Rios, G ;
Mulet, JM ;
Serrano, R ;
de Larrinoa, IF .
FEBS LETTERS, 1998, 425 (02) :323-328
[64]   Promoter sequences regulated by the calcineurin-activated transcription factor Crz1 in the yeast ENA1 gene [J].
Mendizabal, I ;
Pascual-Ahuir, A ;
Serrano, R ;
de Larrinoa, IF .
MOLECULAR GENETICS AND GENOMICS, 2001, 265 (05) :801-811
[65]   Activated calcineurin confers high tolerance to ion stress and alters the budding pattern and cell morphology of yeast cells [J].
Mendoza, I ;
Quintero, FJ ;
Bressan, RA ;
Hasegawa, PM ;
Pardo, JM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (38) :23061-23067
[66]  
MENDOZA I, 1994, J BIOL CHEM, V269, P8792
[67]  
MINET M, 1992, PLANT J, V2, P417
[68]   The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance [J].
Miyahara, K ;
Mizunuma, M ;
Hirata, D ;
Tsuchiya, E ;
Miyakawa, T .
FEBS LETTERS, 1996, 399 (03) :317-320
[69]  
MORAN JF, 1994, PLANTA, V194, P346, DOI 10.1007/BF00197534
[70]  
Mulet JM, 1999, MOL CELL BIOL, V19, P3328