PCR detection of cows' milk in water buffalo milk and mozzarella cheese

被引：79

作者：

López-Calleja, I ^{[1
]}

Alonso, IG ^{[1
]}

Fajardo, V ^{[1
]}

Rodríguez, MA ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

INTERNATIONAL DAIRY JOURNAL | 2005年 / 15卷 / 11期

关键词：

PCR; cow; water buffalo; mozzarella cheese; 12S rRNA gene;

D O I：

10.1016/j.idairyj.2004.12.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Polymerase chain reaction (PCR) has been applied for the specific detection of cows' DNA in water buffalo milk and mozzarella cheese by using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of specific primers for cow yielded a 346 bp fragment from cows' milk DNA, whereas no amplification signal was obtained in sheep's, goats' and water buffalo's milk DNA. Analysis of both buffalo milk and buffalo mozzarella cheese mixtures containing different percentages of cows' milk or bovine mozzarella cheese, enabled the specific detection of cow's DNA with a sensitivity threshold of 0.1%. The proposed PCR assay represents a rapid and straightforward method for the detection of adulterations in water buffalo milk and mozzarella cheese. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1122 / 1129

页数：8

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