Protein-functionalized polymer brushes

被引：187

作者：

Tugulu, S

Arnold, A

Sielaff, I

Johnsson, K

Klok, HA

机构：

[1] Ecole Polytech Fed Lausanne, Lab Polymeres, Inst Mat, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Ingn Prot Lab, Inst Sci & Ingn Chim, CH-1015 Lausanne, Switzerland

来源：

BIOMACROMOLECULES | 2005年 / 6卷 / 03期

关键词：

D O I：

10.1021/bm050016n

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O-6-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O-6-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.

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页码：1602 / 1607

页数：6

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