Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases

被引:81
作者
Guo, HM
Majmudar, G
Jensen, TC
Biswas, C
Toole, BP
Gordon, MK [1 ]
机构
[1] Tufts Univ, Sch Med, Dept Anat & Cellular Biol, Boston, MA 02111 USA
[2] Mary Kay Holding Co, Dallas, TX USA
[3] Aastrom Biosci Inc, Ann Arbor, MI USA
关键词
tumor cell-derived collagenase stimulatory factor; basigin; M6; CD147;
D O I
10.1016/S0378-1119(98)00400-4
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
EMMPRIN (extracellular matrix metalloproteinase inducer) also known as CD147 and basigin, is a member of the immunoglobulin family that is present on the surface of tumor cells and stimulates nearby fibroblasts to synthesize matrix metalloproteinases. Using our EMMPRIN cDNA, we have isolated a cosmid clone that contains the human EMMPRIN gene. S1 analysis with a fragment of the gene clone and primer extension of the mRNA was performed to determine the transcription start site. PCR and sequence analysis have defined the exon/intron organization of the gene and show that it is highly conserved with the mouse EMMPRIN/basigin gene. About 950 bases of the 5'-flanking region were examined for transcription factor consensus binding sites, locating three SP1 sites and two AP2 sites. The transcription start site was found to be located in a CpG island. Elements in the proximal promoter region were conserved in the human and mouse genes. (C) 1998 Elsevier Science B.V. All rights reserved.
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页码:99 / 108
页数:10
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